Molecular Characterization Of Non-Fermenting Gram-Negative Bacilli And Its Virulence Markers From Various Clinical Samples In A Tertiary Care Center, South India
نویسندگان
چکیده
Introduction: Non-fermentative Gram-negative bacilli (NFGNB) are an important cause of healthcare-associated infections, and theyare primarily opportunistic pathogens.Aim objectives: To isolate characterize the non-fermenting from various clinical samples ina tertiary care center. detect virulence markers drug resistance using molecular methods.Material methods: were identified standardbacteriological identification methods. Conventional PCR for detection MBLs genes P. aeruginosa such as blaIMP, blaVIM, blaNDMand a marker, Exo S gene also MBL A. baumannii blaNDM, blaOXA-23, blaOXA-58, avirulence OmpA gene.Results: Out 104 NFGNB isolates Pseudomonas was most common bacteria comprising 62% (n=65), followed byAcinetobacter 25% (n=26), Acinetobacter lwoffii 4% (n=4), flourescens 3% (n=3), Burkholderia cepacia 3%(n=3), Stenotrophomonas maltophilia 1% (n=1), Myroides odoratimimus Rhizobium radiobacter (n=1). The resultof showed that blaVIM expressed in 25 %(n=2), blaNDM 50 %(n=4) blaIMPwas found %(n=2). For baumannii, 33% (n=6), blaOXA-23 78%(n=14), blaOXA-58 56% (n=10) with no expression blaIMP genes.Conclusion: In our study we report increased prevalence baumannii. This highlights theimportance accurate producers judicious use carbapenems. Early continuedsurveillance these multidrug resistant organisms will help to prevent their spread hospital environment.
منابع مشابه
Prevalence and antimicrobial susceptibility of non-fermenting Gram-negative bacilli isolated from clinical samples at a tertiary care hospital.
INTRODUCTION: We compared the prevalence and antimicrobial susceptibility of non-fermenting gram-negative bacilli (NFGNB) isolated from clinical samples at a Brazilian tertiary care hospital in 2008 and 2013. METHODS: Collected data included patient's name, age, sex, inpatient unit, laboratory record number, type of biological material, culture test result, and antimicrobial susceptibility of...
متن کاملIsolation and Identification of Non Fermenting Gram Negative Bacilli in A Tertiary Care Hospital
Non-Fermenting Gram-Negative Bacilli (NFGNB )group includes numerous organisms but the ones which are known to cause nosocomial infections are Pseudomonas aeruginosa, Acinetobacter baumanii, Burkholderia cepacia complex (BCC) and Stenotrophomonas maltophila .This study was undertaken to identify the various nonfermenters isolated from patients admitted to our hospital, a tertiary care center, a...
متن کاملSpectrum of Non fermenting gram negative bacilli infection (excluding Pseudomonads ) in tertiary care hospital
متن کامل
Phenotypic Characterization and Antibiotic Susceptibility Pattern of Non-fermentative Gram Negative Bacilli from Clinical Samples
Non fermenting gram negative bacteria were considered to be nonpathogenic and commensal of little significance. Recently there has been a tremendous interest in these organisms as they are being isolated from clinical specimens with increasing frequency. So this study aims at isolation, identification and antibiotic susceptibility of non-fermenting gram negative bacilli from various clinical sp...
متن کاملDetection of Metallo-Beta-lactamase Production in Rare Carbapenem-Resistant Non-fermentative Gram-Negative Bacilli Isolated in a Tertiary Care Hospital, Visakhapatnam, India
Introduction: Non-fermentative Gram-negative bacilli (NFGNB) are occasionally involved in infectious diseases pathology, but have shown resistance to multiple antibiotics and the capability to gain new resistance factors in the hospital environment. The present study was aimed to investigate the antibiotic susceptibility pattern of rare NFGNB isolated from different clinical samples and the pre...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Pharmaceutical Negative Results
سال: 2022
ISSN: ['0976-9234', '2229-7723']
DOI: https://doi.org/10.47750/pnr.2022.13.s05.242